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                                    296 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGY3.2. Suitability of different solid substrates for pigment productionThe study highlights the significant impact of substrate type on pigment yield in solid-state fermentation using M. purpureusHG12. Among the tested substrates, rice bran demonstrated the highest yield (4.627 %u00b1 0.03 O.D/g dry substrate), likely due to its superior nutrient content and porous structure, which support fungal growth and pigment synthesis. Plain rice showed moderate yield (2.627 %u00b1 0.03), while red rice had the lowest (1.90 %u00b1 0.03), possibly due to its dense structure and lower nutrient availability. These findings underscore the potential of rice bran as an optimal and cost-effective substrate for industrial-scale natural pigment production.Table 1. Effect of different substrates on pigment yield (O.D/g dry substrate) by solid fermentation grown culture of Monascus purpureus HG12Substrate Pigment yield(O.D/g dry substrate)Plain rice 2.627%u00b10.03Red rice 1.90%u00b10.03Rice bran 4.627%u00b10.03 (a) (b) (c)Figure 2. The pigment formation of the M. purpureus HG12 strain after 20 days of cultivation on substrates such as plain rice (a), red rice (b), and rice bran (c)
                                
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