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SECTION I: MOLECULAR GENETIC ENGINEERING AND BIOCHEMICAL TECHNOLOGY 183CS, PEG, and PLA in the nanofibers, which caused certain shifts in the absorption peaks of the functional groups. Mechanical analysis of the nanofibers exhibited a tensile strength of 2.11 %u00b1 0.64 MPa, an elongation at break of 13.22 %u00b1 1.65%, and a Young%u2019s modulus of 95.49%u00b14.94 MPa. The low tensile strength of the fiber membrane may explain the reason chitosan is a polymer with low mechanical strength. Wettability is a fundamental property that significantly impacts drug release mechanisms, influencing dissolution rates, release kinetics, formulation design, and overall therapeutic efficacy. Understanding and controlling wettability is essential for the development of effective pharmaceutical products [27]. The nanofibers were found to be hydrophilic with the contact angle of 73.7 %u00b1 1.5o. The presence of PEG in the nanofibers is considered to be responsible for the hydrophilicity of nanofibers CS/PLA/PEG loaded capsaicin. The nanofibers exhibited antibacterial activity against four types of microorganisms: Escherichia coli ATCC 25922 (MIC value of 100 %u00b5g/mL),Staphylococcus aureus subsp. aureus ATCC 25923 and Aspergillus niger ATCC 6275 (MIC value of 200 %u00b5g/mL), Bacillus subtillis subsp. spizizenii ATCC 6633 (MIC value of 400 %u00b5g/mL). The results obtained in Table 1 show that nanofibers exhibited better antibacterial activity against Escherichia coli and Staphylococcus aureus than free capsaicin. Perhaps CS, an antibacterial polymer also contributed to the antibacterial effect of the nanofibers CS/PLA/PEG loaded capsaicin. The antibacterial effect of the nanofibers and free capsaicin against strain Aspergillus niger was similar. However, the nanofibers revealed weaker activity against strain Bacillus subtillis subsp. spizizenii than free capsaicin, probably due to the low capsaicin content in the nanofibers leading to a decrease in this activity. Neither the nanofibers nor capsaicin exhibited antibacterial activity against the remaining strains at the test concentrations.