Page 15 - Demo

MINING NOVEL ANTI-TUBERCULOSIS COMPOUNDS FROM ACTINOMYCETES LIBRARYJoo-Won Suh1,2, *Abstract: ECUM, an Extract Collection of Useful Microorganisms, encompasses over 20,000 strain of actinomycetes and more than 200,000 fermentation broth extract. These strains were isolated from ecologically distinctive soil, aiming to isolate unique and uncharacterized metabolites. Through one of this effort, Ecumicin, a novel cyclic peptide compound with strong anti-tuberculosis activity, was isolated from Nonomuraea sp MJM5123 in ECUM library. Rufomycin, another cyclic peptide with strong antituberculosis activity, was also discovered from Streptomyces sp. MJM3502 in this library. Ecumicin and rufomycin are composed of unusual amino acids and have unique mode of action that collapse protein homeostasis through influence of the ATPase and proteolytic activity of ClpC1/ClpP1/ClpP2 complex. The MIC90 of ecumicin and rufomycin was analyzed to be 0.16 and 0.019 %u03bcM against drug susceptible M. tuberculosis, outperforming other compounds targeting M. tuberculosis ClpC1. Notably, Ecumicin and rufomycin showed excellent activity against both multidrug-resistant M. tuberculosis (MDR-TB) and extensively 1 Myongji Bioefficacy Research Center, Myongji University, Yongin, Gyeonggi-do, Republic of Korea; jwsuh@mju.ac.kr2 Microbiohealthcare Co. Ltd. Yongin, Gyeonggi-do, Republic of Korea; jwsuh@mju.ac.kr* Correspondence: jwsuh@mju.ac.kr