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128 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYpercentage of 26.47%, followed by 3-carene of 17.31%, D-limonene of 18.7% and %u03b2-pinene of 9.97%. In conclusion, black pepper in Dak Lak province of Vietnam provides abundant essential oil yield in terms of quality and quantity. This reveals that black pepper sourced in Dak Lak province of Vietnam is a high value essential oil for many useful applications and export.Keywords: Black pepper, Piper nigrum L., Dak Lak province, essential oil extration, bioactive compounds1. INTRODUCTIONBlack pepper has a scientific name as Piper nigrum L., belonging to the Piperaceae family. It is an important and most common spice, called %u201cthe king of spices%u201d and appreciated for both its distinctive aroma and pungency [1]. This plant is popularly grown in tropical countries. During the last 10 years, Vietnam was ranked as the world%u2019s largest producer and exporter of pepper, followed by Indonesia, India, Brazil, Malaysia, Sri Lanka, China, Thailand and Bangladesh [2-4]. In Vietnam, black pepper is cultivated in the Central Highlands and the Southeast regions for production of entire seed and spices in food industry [5]. Pepper contains about 1-4% of essential oil content which are volatile compounds formed by 70% monoterpenes, 25% sesquiterpenes, and 5% oxygenated compounds [5-8]. It is a warm, spicy aroma mainly caused by hydrocarbon monoterpenes such as sabinene, pinene, limonene, terpinene, myrcene, %u03b4-3-carene and monoterpene derivatives (borneol, carvone, carvacrol, 1,8-cineol, linalool) [6-8]. Sesquiterpenes accounts for 20% of black pepper essential oil in which caryophyllene is a natural bicyclic sesquiterpene. Other substances such as %u03b1-humulene, caryophyllene oxide and ketone have been rarely found.