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STUDY ON EXTRACTION AND COMPOSITION ANALYSIS OF ESSENTIAL OIL FROM BLACK PEPPER (PIPER NIGRUM L.) GROWN IN DAK LAK PROVINCE OF VIETNAMThi Cam Van Do1,*, Quang Tung Nguyen1, Dinh Giap Vu1, Thi Thanh Yen Pham2, Thi Huong Dang3 Abstract: Black pepper (Piper nigrum L.) is a medicinal plant grown popularly in Dak Lak province of Vietnam. The plant is evaluated as a high economic value crop due to high content of essential oil and valuable bioactive chemicals. In this study, the 10 L hydro-distillation system was used to investigate the effects of types and sizes of raw materials, distillation time and material-to-water ratio for optimization of oil recovery yield. Data revealed that the maximal oil yield of 2.18% was recovered from the Dak Lak%u2019s black pepper under the optimal conditions: Dry biomass, material to water ratio of 100 g/L, material size of 0.5 mm and distillation time of 240 min. The harvested essential oil was quantitatively analyzed by GC-MS, yielding 16 bioactive compounds identified including caryophyllene with the highest 1 HaUI Institute of Technology, Hanoi University of Industry (HaUI), 298 Cau Dien, Bac Tu Liem, Hanoi, Vietnam; 2 Faculty of Chemical technology, Hanoi University of Industry (HaUI), 298 Cau Dien, Bac Tu Liem, Hanoi, Vietnam; 3 Institute of Biological and Food Technology, Hanoi Open University, B101 Nguyen Hien, Hai Ba Trung, Hanoi, Vietnam; * Correspondence: docamvan85@gmail.com