Page 341 - Demo

SECTION II. APPLIED BIOTECHNOLOGY IN THE PHARMACEUTICAL INDUSTRY... 345rice bran extract or standard. All determinations were performed in three times (n = 3).2.6. ABTS assay An ATBS assay was conducted following the method of Arnao et al [10]. The stock solutions included a 7 mM ABTS solution and a 2.4 mM K2S2O8 solution. Mix two stock solutions with a ratio of 1:1 and keep it for 14 hours to get an ATBS working solution. Then dilute the working solution until absorbance was 0.706 %u00b1 0.01 units at 734 nm. Finally, 10 %u00b5L each sample was mixed with 190 %u03bcL ABTS working solution and measured absorbance at 734 nm. The ABTS free scavenging capacity of the rice bran extract is calculated by the following equation: ABTS radical scavenging activity % = (Abscontrol - Abssample) x 100/ Abscontrolwhere Abscontrol is the absorbance of ABTS radical in methanol; Abssample is the absorbance of ABTS radical solution mixed with sample extract/standard. All determinations were performed in triplicate (n = 3).3. RESULTS 3.1. Screening of lactic acid bacteria with high glycosidase activityTo assess glycosidase activity, %u03b2-glucosidase and %u03b2-galactosidase activities were measured in 8/30 Lactobacillus strains isolated from fermented food (pickled radish, pickled eggplant, pickled mustard greens, pickled shrimp, and kimchi) with the characteristic of probiotics. Lactobacillus rhamnosus GG (LGG) was used as the positive control. The glycosidase activity is shown in Table 1. Among the 8 tested strains, 5/8 strains exhibited both %u03b2-glucosidase and %u03b2-galactosidase activities, 2 strains showed only %u03b2-glucosidase effect and 1 strain did not show