Page 357 - Demo

SECTION II. APPLIED BIOTECHNOLOGY IN THE PHARMACEUTICAL INDUSTRY... 361Dextrose Agar (PDA) as the culture medium, prepared at a ratio of 39 grams of PDA to 1000 mL of distilled water. The solution is boiled and sterilized at 125%u00b0C for 15 minutes to support the growth and counting of mold and yeast in the sample. The total mold and yeast count is calculated using the following formula: N = log[(C x d)/ V]Where: N is total viable count (log (CFU)/g); C represents the total count of mold and yeast colonies on the agar plate.; V represents the volume of the sample solution analyzed; d is the sample dilution factor.2.3.5. Aerobic bacteria countThe method for culturing and determining the total aerobic bacteria count follows AOAC standard 966.23. This method uses Plate Count Agar (PCA) as the culture medium, prepared at a ratio of 23.5 grams of PCA to 1000 mL of distilled water. The solution is boiled and sterilized at 125%u00b0C for 15 minutes to support the growth and counting of microorganisms in the sample. The total aerobic microbial count is calculated using the following formula:N = log[(C x d)/ V]Where: N is total viable count (log (CFU)/g); C represents the total count of aerobic bacteria colonies on the agar plate; V represents the volume of the sample solution analysed; d is the sample dilution factor.2.3.6. Tea Colour/Total Liqour ColorThe method is adapted from the research conducted by Kevin L. Goodner et al. in 2008 with modifications [6]. To prepare the samples, prepare 5 mL of 0.3 %u00b0Brix tea by shaking the tea for 10 minutes and filter the liquid. Take 5 ml of the brewed tea and dilute it with 45 ml of water at a 1:10 ratio. Measure the diluted