Page 273 - Demo

EFFECTS OF MEDIUM COMPONENTS ON THE PRODUCTION OF VITAMIN K2 BY BACILLUS SUBTILIS BST2Son Huy Dinh1, Huong Thu Dang1*, Anh Tu Loc1, Anh La Nguyen1Abstract: Vitamin K2, an essential lipid-soluble vitamin, plays a critical role in blood clotting, prevention of osteoporosis, and cardiovascular disease, and has become a focus of research in recent years. This study aimed to evaluate the capacity of Bacillus subtilis strains isolated from fermented foods to produce vitamin K2 and to determine the optimal combination of key nutrients to enhance its yield. Screening of 14 Bacillus subtilis strains revealed that strains NTNS1, BST1, and BST2 are promising vitamin K2 producers, with yields of 737.0, 936.1, and 1170.5 %u00b5g/g of lyophilized cells, respectively. Further investigation, focusing on strain BST2, indicated that the optimal medium components for vitamin K2 production were 70 g/L glycerol, 20 g/L soybean peptone, and 0.5 g/L K2HPO4. Under these conditions, this strain produced a maximum menaquinone concentration of 2694.5 %u00b5g/L after 120 hours of fermentation.Keywords: Bacillus subtilis, fermentation, menaquinones, vitamin K2.1 Food Industries Research Institute, 301 Nguyen Trai road, Thanh Xuan district, Hanoi, Vietnam* Correspondence: huongdt@firi.vn