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SECTION I: MOLECULAR GENETIC ENGINEERING AND BIOCHEMICAL TECHNOLOGY 203 (a) (b)Figure 5. Color reaction (MTT) of strain N1 AIA6 when testing for cancer cell inhibitory activity (N1 AIA6 color reaction with normal cells HEK 293T (a) and with liver cancer cells Hep G2 (b)3.3. Identification results of 3 selected highly active strainsThree highly active bacterial isolates, N2 GII14, N6 GII4, and N1 AIA6, were selected for identification by 16S rRNA gene sequence analysis (Figure 6). The total DNA of the three strains was extracted, PCR, and electrophoresed on 1% agarose gel (Figure 7). PCR products of all three genes gave a clear, specific band, approximately 1.5 kb in size, corresponding to theoretical calculations. The PCR products were further purified (PCR product purification kit, Invitrogen) and sequenced (ABI PRISM 3100, Applied Bioscience). The received raw sequences were processed using Bioedit software and compared with other gene sequences in the NCBI gene bank using the BLAST program. The results showed that strain N2 GII14 was 99.36% identical to the 16S rRNA gene of Bacillus amyloloquefaciens strain HSAM5 (Ass.No MT258998.1), strain N6 GII4 was 100% identical to the 16S rRNA gene of Bacillus siamensis strain BSi MA22 (Ass.No ON878257.1), strain N1 AIA6 was 99.78% identical to the 16S